AIMS Medical Science | |
Angiotensin II induces the secretion of ICAM-1 and MCP-1 in human airway smooth muscle cells in vitro | |
article | |
Ning Li1  Yating Huo2  Haojun Xie2  Yuanxiong Cheng1  | |
[1] Department of Neurosurgery, The First Affiliated Hospital, Jinan University;Department of Respiratory Disease, The Third Affiliated Hospital, Southern Medical University | |
关键词: human airway smooth muscle cells; angiotensin II; ICAM-1; MCP-1; NF-κB signal pathway; ; | |
DOI : 10.3934/medsci.2018.3.259 | |
来源: American Institute of Mathematical Sciences | |
【 摘 要 】
Background: Angiotensin [1] II is known to cause human airway smooth muscle (HASM) contraction and closely relate to asthma, however, the effect of Ang II on HASM cells (HASMCs) secretion function is still unclear. Methods: Primary cultured HASMCs were treated with Ang II, Ang II + Ang-(1–7), and Ang II + irbersatan (IRB), respectively. The nuclear translocation of the NF-κB p65 in HASMCs in each group was analyzed performed with Immunofluorescence. The expression levels of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) were evaluated by Real-time polymerase chain reaction (PCR) and Enzyme linked immunosorbent assay (ELISA). Results: Ang II caused sharply increase of the nuclear translocation of NF-κB p65, also, Ang II significantly increased expression of ICAM-1 and MCP-1 secreted by HASMCs, this effect could be inhibited by Ang-(1–7) and IRB. Conclusion: These data may indicate the effect of Ang II on inducing HASMCs to secret ICAM-1 and MCP-1, which might be through the NF-κB p65 pathway by AT-1 receptor.
【 授权许可】
CC BY
【 预 览 】
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