Mechanisms mediating the antiproliferative effects of nitric oxide in cultured human airway smooth muscle cells

FEBS Letters

Knox, Alan J¹  Hamad, Ahmed M² 

[1] Department of Respiratory Medicine, City Hospital, Hucknall Road, Nottingham NG5 1PB, UK;Department of Respiratory Medicine, Al-Mansourah University Hospital, Al-Gomhoria St., Al-Mansourah, Egypt

关键词: Nitric oxide;    cGMP;    Smooth muscle;    Remodelling;    Asthma;    Cell cycle;    HASMC;    human airway smooth muscle cells;    SNAP;    S-nitroso-N-acetyl penicillamine;    Hb;    haemoglobin;    MB;    methylene blue;    PDE;    phosphodiesterase;    FBS;    foetal bovine serum;   

DOI  :  10.1016/S0014-5793(01)02883-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have characterised the mechanisms involved in the antiproliferative effect of NO in human airway smooth muscle cells (HASMC). S-Nitroso-N-acetyl penicillamine, a nitric oxide donor, inhibited proliferation in both G₁ and S phases of the cell cycle. Additionally, experiments with 8-bromo-cGMP, haemoglobin, a NO scavenger and zaprinast, a cGMP-specific phosphodiesterase inhibitor, showed that both effects were NO-mediated. The G₁ phase inhibition was cGMP-dependent whereas the S phase inhibition was due to a cGMP-independent inhibition of ribonucleotide reductase. These results demonstrate that NO inhibits HASMC proliferation by cGMP-dependent and -independent mechanisms acting at distinct points in the cell cycle.

【 授权许可】

Unknown