FEBS Letters | |
Mechanisms mediating the antiproliferative effects of nitric oxide in cultured human airway smooth muscle cells | |
Knox, Alan J1  Hamad, Ahmed M2  | |
[1] Department of Respiratory Medicine, City Hospital, Hucknall Road, Nottingham NG5 1PB, UK;Department of Respiratory Medicine, Al-Mansourah University Hospital, Al-Gomhoria St., Al-Mansourah, Egypt | |
关键词: Nitric oxide; cGMP; Smooth muscle; Remodelling; Asthma; Cell cycle; HASMC; human airway smooth muscle cells; SNAP; S-nitroso-N-acetyl penicillamine; Hb; haemoglobin; MB; methylene blue; PDE; phosphodiesterase; FBS; foetal bovine serum; | |
DOI : 10.1016/S0014-5793(01)02883-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have characterised the mechanisms involved in the antiproliferative effect of NO in human airway smooth muscle cells (HASMC). S-Nitroso-N-acetyl penicillamine, a nitric oxide donor, inhibited proliferation in both G1 and S phases of the cell cycle. Additionally, experiments with 8-bromo-cGMP, haemoglobin, a NO scavenger and zaprinast, a cGMP-specific phosphodiesterase inhibitor, showed that both effects were NO-mediated. The G1 phase inhibition was cGMP-dependent whereas the S phase inhibition was due to a cGMP-independent inhibition of ribonucleotide reductase. These results demonstrate that NO inhibits HASMC proliferation by cGMP-dependent and -independent mechanisms acting at distinct points in the cell cycle.
【 授权许可】
Unknown
【 预 览 】
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RO201912020310995ZK.pdf | 342KB | download |