PIWIL2 and PL2L60 (Cancer/Testis genes) Expression in Breast Cancer

Archives of Breast Cancer

article

Rezvan Esmaeili¹  Keivan Majidzadeh-A¹  Maryam Ghasemi²  Leila Farahmand¹  Nasrin Abdoli¹ 

[1] Breast Cancer Genetics Department, Breast Cancer Research Center, ACECR;Tasnim Biotechnology Research Center (TBRC), school of medicine, AJA University of Medical Sciences

关键词: PIWIL2;    PL2L60;    cancer/testis antigens;    breast cancer;   

DOI  :  10.19187/abc.20141391-94

学科分类：社会科学、人文和艺术（综合）

来源: Kaviani Breast Disease Institute

【 摘 要 】

Background: Cancer/testis antigens ( s) are members of a group of CTA proteins which are normally expressed in testis germ cells and to a lesser extent in the ovaries. Because of recent reports about their aberrant and specific expression in some tumoral tissues, they may play a role as new candidates for targeted therapy. Therefore, the study of the expression pattern of these biomarkers and its relationship with clinical features of the patients is a subject of great interest. Methods: In this study, expression of and genes was studied by PIWIL2 multiplex - in 65 breast tissue samples including 30 invasive ductal RT PCR carcinomas ( ), 30 normal adjacent tissue samples and five normal breast tissue IDC samples and 2 normal testicular tissue samples as positive controls. beta actin was considered as internal control. Results: Results of gel electrophoresis analysis demonstrated no significant expression of target genes in any sample except testis. Simultaneously, beta actin was expressed in all the samples. Conclusions: The present study indicates lack of and PIWIL PL 2 2L60 expression at m level in breast cancer. Although cell lines can be used in RNA cancer research, they are not representative of tumor tissues. More studies investigating the expression of these genes at protein level will help us decide whether to apply these candidate genes as tumor markers or not.

【 授权许可】

CC BY-NC