Long-term case study of a Wuzhishan miniature pig with diabetes

Animal Models and Experimental Medicine

article

Miaomiao Niu¹  Yaqian Liu¹  Lei Xiang¹  Yuqiong Zhao¹  Jifang Yuan¹  Yunxiao Jia¹  Xin Dai¹  Hua Chen¹ 

[1] Laboratory Animal Center, Chinese PLA General Hospital;State Key Laboratory of Kidney Diseases, Chinese PLA General Hospital

关键词: beta-cell regeneration;    diabetes-related complications;    pig;   

DOI  :  10.1002/ame2.12098

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Background: Miniature pigs are attractive animal models for exploring diabetes because they are similar to humans in terms of physiological structure and metabolism. However, little is known about the complications of diabetes in pigs. Methods: In this study, a 28-month observation of a Wuzhishan miniature pig with streptozotocin (STZ)-induced (120 mg/kg) diabetes was conducted, to investigate diabetes-related complications and the possibility of self-recovery in miniature pigs. Blood glucose, serum and urinary biochemistry was measured, and histopathologic examinations of eyes, kidney and pancreas were made. Results: During the observation, diabetic complications of eyes and kidney were observed. The eye complications included bilateral cataracts in the 15th month and degeneration of inner retina and microaneurysm in the 28th month. Kidney complications included glomerular mesangial expansion, focal segmental glomerular sclerosis, and renal tubular epithelial degeneration, but no proteinuria was observed. By 28 months after the application of STZ, with no treatment given, blood glucose had recovered and the number of pancreatic islet beta-cells had increased significantly. Conclusions: We showed that the STZ-induced diabetes model in miniature pigs could accurately mimic the pathological changes of human diabetes, and that pancreatic islet beta-cell regeneration did occur in an adult miniature pig, providing a new means for exploring diabetic complications and pancreatic islet beta-cell regeneration.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND