Radiosensitization effect of selenocystine on HeLa cells invitro

Precision Radiation Oncology

article

Shen Qian¹  Li-xin Xie¹  Guo-bao Li²  Sha-sha Zheng²  Na Li¹ 

[1] Department of Respiratory and Critical Care Medicine, the First Medical Center, Chinese PLA General Hospital;The Third Department of Pulmonary Disease, The Third People's Hospital of Shenzhen

关键词: apoptosis;    cervical cancer;    HeLa cell;    radiosensitization;    selenocystine;   

DOI  :  10.1002/pro6.1085

学科分类：社会科学、人文和艺术（综合）

来源: Wiley

【 摘 要 】

Objectives: To study the effect of selenocystine (Selenocystine, SeC) on the radiosensitivity of human cervical cancer Hela cells, and to provide a new target for radiosensitivity of cervical cancer. Methods: SeC were added to the cervical cancer Hela cells, and then irradiate with 200 cGy/min X-rays. The radiosensitization effect of SeC on cervical cancer Hela cells were confirmed with the experiments of cell growth inhibition, clone formation, flow cytometry and Western blotting to detect apoptosis. Results: SeC were added to the Hela cells at different doses of 0, 5, 10, and 20 ?mol/L and incubating for 24 hours, the cell growth inhibition rates were: 0%, 22.68%, 41.71%, 74.19%, and the calculated IC50 was 14.83 ?mol/L. The growth inhibition rate of Hela cells was dose-dependent with the concentration of SeC. The results of the colony formation experiments showed that the cell colony formation rates of the single X-ray irradiation doses of 0, 2, 4, 6, and 8 Gy were 100%, 77.56%, 46.89%, 28.67%, and 10.89%, respectively. After adding 5?mol/L SeC under the same irradiation dose, the colony formation rate was reduced to 70.98%, 59.45%, 30.67%, 14.29%, 3.72%; 5?mol/L SeC combined with radiation therapy can shift the survival curve to the left, and the D0 and Dq values were significantly smaller than those in the radiation group, P < 0.05. Hela cell apoptosis were detected by the flow cytometry showed that when the single X-ray irradiation dose was 0, 2, 4, 8 Gy, the apoptosis scores were: 2.51±0.06, 6.76±0.03, 14.51±0.15, 21.41±0.08;When 5 ?mol/L SeC was added and then combined with X-ray irradiation, and the irradiation dose was 0, 2, 4, 8Gy, the apoptosis score of each group was: 10.89 ± 0.36, 15.18 ± 0.21, 31.19 ± 0.08, 45.53 ± 0.28, SeC combined with X-ray had an interaction effect on Hela cells, and the apoptosis rate increased (F = 21.52, P < 0.01). When Hela cells were treated with SeC and X-rays, the expression of PARP protein and phosphorylated p53 protein was significantly increased compared with the control group. Conclusions: SeC may have a radiosensitizing effect on Hela cells, and its mechanism may be related to promoting apoptosis. SeC will be expected to become a new sensitization target for cervical cancer radiotherapy.

【 授权许可】

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