Lymphocyte-specific protein 1 (LSP1) regulates bone marrow stromal cell antigen 2 (BST-2)-mediated intracellular trafficking of HIV-1 in dendritic cells

FEBS Letters

article

Rutuja Kulkarni¹  Shuxian Jiang¹  Gabriel Birrane¹  Anil Prasad¹ 

[1] Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School

关键词: cytoskeleton;    dendritic cell;    endocytosis;    human immunodeficiency virus;    trafficking;   

DOI  :  10.1002/1873-3468.13788

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human immunodeficiency virus type 1 (HIV-1) subverts intracellular trafficking pathways to avoid its degradation and elimination, thereby enhancing its survival and spread. The molecular mechanisms involved in intracellular transport of HIV-1 are not yet fully defined. We demonstrate that the actinbinding protein lymphocyte-specific protein 1 (LSP1) interacts with the interferon-inducible protein bone marrow stromal antigen 2 (BST-2) in dendritic cells (DCs) to facilitate both endocytosis of surface-bound HIV-1 and the formation of early endosomes. Analysis of the molecular interaction between LSP1 and BST-2 reveals that the N terminus of LSP1 interacts with BST-2. Overall, we identify a novel mechanism of intracellular trafficking of HIV-1 in DCs centering on the LSP1/BST-2 complex. We also show that the HIV1 accessory protein Vpu subverts this pathway by inducing proteasomal degradation of LSP1, augmenting cell–cell transmission of HIV-1.

【 授权许可】

Unknown