Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals

Cells

Andrius Masedunskas¹  Natalie Porat-Shliom¹  Kamil Rechache¹  Myo-Pale’ Aye¹ 

[1] Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, Bethesda 20892-4340, MD, USA;

关键词: intravital microscopy;    in vivo imaging;    endocytosis;    cytoskeleton;    salivary glands;   

DOI  :  10.3390/cells1041121

来源: mdpi

【 摘 要 】

Intravital microscopy has enabled imaging of the dynamics of subcellular structures in live animals, thus opening the door to investigating membrane trafficking under physiological conditions. Here, we sought to determine whether the architecture and the environment of a fully developed tissue influences the dynamics of endocytic processes. To this aim, we imaged endocytosis in the stromal cells of rat salivary glands both in situ and after they were isolated and cultured on a solid surface. We found that the internalization of transferrin and dextran, two molecules that traffic via distinct mechanisms, is substantially altered in cultured cells, supporting the idea that the three dimensional organization of the tissue and the cues generated by the surrounding environment strongly affect membrane trafficking events.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.