Overexpressed β-cyanoalanine synthase functions with alternative oxidase to improve tobacco resistance to salt stress by alleviating oxidative damage

FEBS Letters

article

Lu-Lu Yu¹  Yang Liu¹  Cui-Jiao Liu¹  Feng Zhu²  Zheng-Quan He³  Fei Xu¹ 

[1] Applied Biotechnology Center, Wuhan University of Bioengineering;College of Horticulture and Plant Protection, Yangzhou University;The Key Laboratory of Plant Genetics Development and Germplasm Innovation in the Three Gorges Region, Biotechnology Research Center, China Three Gorges University

关键词: alternative oxidase;    cyanide;    Nicotiana tabacum;    respiration;    salt stress;    b-cyanoalanine synthase;   

DOI  :  10.1002/1873-3468.13723

来源: John Wiley & Sons Ltd.

【 摘 要 】

b-Cyanoalanine synthase (b-CAS) is an enzyme involved in cyanide detoxification. However, little information is available regarding the effects of b-CAS activity changes on plant resistance to environmental stress. Here, we found that b-CAS overexpression (CAS-OE) improves the resistance of tobacco plants to salt stress, whereas plants with b-CAS silencing suffer more oxidative damage than wild-type plants. Notably, blocking respiration by the alternative oxidase (AOX) pathway significantly aggravates stress injury and impairs the salt stress tolerance mediated by CAS-OE. These findings present novel insights into the synergistic effect between b-CAS and AOX in protecting plants from salt stress, where b-CAS plays a vital role in restraining cyanide accumulation, and AOX helps to alleviate the toxic effect of cyanide.

【 授权许可】

Unknown