Liquid–liquid phase separation of the Golgi matrix protein GM130

FEBS Letters

article

Aleksander A. Rebane¹  Pascal Ziltener¹  Lauren C. LaMonica¹  Antonia H. Bauer¹  Hong Zheng¹  Iván López-Montero²  Frederic Pincet¹  James E. Rothman¹  Andreas M. Ernst¹ 

[1] Department of Cell Biology, Yale School of Medicine;Universidad Complutense de Madrid;Instituto de Investigación Hospital Doce de Octubre (i+12);Laboratoire de Physique de l’Ecole Normale Supérieure, PSL Research University, Université Paris Diderot Sorbonne Paris Cité, Sorbonne Universités UPMC Univ

关键词: coiled-coil;    Golgi matrix;    Golgin;    liquid;    liquid phase separation;   

DOI  :  10.1002/1873-3468.13715

来源: John Wiley & Sons Ltd.

【 摘 要 】

Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50–400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.

【 授权许可】

Unknown