Measurement of the redox state of the plastoquinone pool in cyanobacteria

FEBS Letters

article

Sergey Khorobrykh¹  Tatsuhiro Tsurumaki²  Kan Tanaka²  Taina Tyystjärvi¹  Esa Tyystjärvi¹ 

[1] Department of Biochemistry/Molecular Plant Biology, University of Turku;Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology;Graduate School of Life Science and Technology, Tokyo Institute of Technology

关键词: cyanobacteria;    plastoquinone;    Synechocystis sp;    PCC6803;   

DOI  :  10.1002/1873-3468.13605

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here, we developed a method for measuring the in vivo redox state of the plastoquinone (PQ) pool in the cyanobacteria Synechocystis sp. PCC 6803. Cells were illuminated on a glass fiber filter, PQ was extracted with ethyl acetate and determined with HPLC. Control samples with fully oxidized and reduced photoactive PQ pool were prepared by far-red and high light treatments, respectively, or by blocking the photosynthetic electron transfer chemically before or after PQ in moderate light. The photoactive pool comprised 50% of total PQ. We find that the PQ pool of cyanobacteria behaves under light treatments qualitatively similarly as in plant chloroplasts, is less reduced during growth under high than under ambient CO2 and remains partly reduced in darkness.

【 授权许可】

Unknown