FEBS Letters | |
Mitochondrial Ca 2+ concentrations in live cells: quantification methods and discrepancies | |
article | |
Celia Fernandez-Sanz1  Sergio De la Fuente1  Shey-Shing Sheu1  | |
[1] Center for Translational Medicine, Department of Medicine, Thomas Jefferson University | |
关键词: fluorescent Ca2+ indicators; genetically encoded Ca2+ indicators; live cells; mitochondrial Ca2+ concentrations; | |
DOI : 10.1002/1873-3468.13427 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Intracellular Ca2+ signaling controls numerous cellular functions. Mitochondria respond to cytosolic Ca2+ changes by adapting mitochondrial functions and, in some cell types, shaping the spatiotemporal properties of the cytosolic Ca2+ signal. Numerous methods have been developed to specifically and quantitatively measure the mitochondrial-free Ca2+ concentrations ([Ca2+ ]m), but there are still significant discrepancies in the calculated absolute values of [Ca2+ ]m in stimulated live cells. These discrepancies may be due to the distinct properties of the methods used to measure [Ca2+ ]m, the calcium-free/bound ratio, and the cell-type and stimulus-dependent Ca2+ dynamics. Critical processes happening in the mitochondria, such as ATP generation, ROS homeostasis, and mitochondrial permeability transition opening, depend directly on the [Ca2+ ]m values. Thus, precise determination of absolute [Ca2+ ]m values is imperative for understanding Ca2+ signaling. This review summarizes the reported calibrated [Ca2+ ]m values in many cell types and discusses the discrepancies among these values. Areas for future research are also proposed.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO202105310000169ZK.pdf | 184KB | download |