Biocompatible Electrochemical Sensor Based on Platinum-Nickel Alloy Nanoparticles for In Situ Monitoring of Hydrogen Sulfide in Breast Cancer Cells

Nanomaterials

Udesh Dhawan¹  Xinke Liu²  Ren-Jei Chung³  Asit Kumar Panda³  Rajalakshmi Sakthivel³  Murugan Keerthi³ 

[1] Centre for the Cellular Microenvironment, University of Glasgow, Glasgow G12 8QQ, UK;College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China;Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei 10608, Taiwan;

关键词: hydrogen sulfide;    endogenous gasotransmitter;    PtNi alloy;    live cells;    electrochemical sensor;   

DOI  :  10.3390/nano12020258

来源: DOAJ

【 摘 要 】

Hydrogen sulfide (H₂S), an endogenous gasotransmitter, is produced in mammalian systems and is closely associated with pathological and physiological functions. Nevertheless, the complete conversion of H₂S is still unpredictable owing to the limited number of sensors for accurate and quantitative detection of H₂S in biological samples. In this study, we constructed a disposable electrochemical sensor based on PtNi alloy nanoparticles (PtNi NPs) for sensitive and specific in situ monitoring of H₂S released by human breast cancer cells. PtNi alloy NPs with an average size of 5.6 nm were prepared by a simple hydrothermal approach. The conversion of different forms of sulfides (e.g., H₂S, HS⁻, and S²⁻) under various physiological conditions hindered the direct detection of H₂S in live cells. PtNi NPs catalyze the electrochemical oxidation of H₂S in a neutral phosphate buffer (PB, pH 7.0). The PtNi-based sensing platform demonstrated a linear detection range of 0.013–1031 µM and the limit of detection was 0.004 µM (S/N = 3). Moreover, the PtNi sensor exhibited a sensitivity of 0.323 μA μM⁻¹ cm⁻². In addition, the stability, repeatability, reproducibility, and anti-interference ability of the PtNi sensor exhibited satisfactory results. The PtNi sensor was able to successfully quantify H₂S in pond water, urine, and saliva samples. Finally, the biocompatible PtNi electrode was effectively employed for the real-time quantification of H₂S released from breast cancer cells and mouse fibroblasts.

【 授权许可】

Unknown