Why the c-Fos/c-Jun complex is extremely conserved: An invitro evolution exploration by combining cDNA display and proximity ligation

FEBS Letters

article

Shou-Kai Kang¹  Xin-Yi Chu¹  Tian Tian¹  Peng-Fei Dong¹  Bai-Xue Chen¹  Hong-Yu Zhang¹ 

[1] Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University

关键词: cDNA display;    c-Fos/c-Jun;    evolution;    proximity ligation;    transcription factor;   

DOI  :  10.1002/1873-3468.13388

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcriptional regulation involves a series of sophisticated protein–protein and protein–DNA interactions (PPI and PDI). Some transcriptional complexes, such as c-Fos/c-Jun and their binding DNA fragments, have been conserved over the past one billion years. Considering the thermodynamic principle for transcriptional complex formation, we hypothesized that the cFos/c-Jun complex may represent a thermodynamic summit in the evolutionary space. To test this, we invented a new method, termed One-Pot-seq, which combines cDNA display and proximity ligation to analyse PPI/PDI complexes simultaneously. We found that the wild-type c-Fos/c-Jun complex is indeed the most thermodynamically stable relative to various mutants of cFos/c-Jun and binding DNA fragments. Our method also provides a universal approach to detect transcriptional complexes and explore transcriptional regulation mechanisms.

【 授权许可】

Unknown