FEBS Letters | |
Why the c-Fos/c-Jun complex is extremely conserved: An invitro evolution exploration by combining cDNA display and proximity ligation | |
article | |
Shou-Kai Kang1  Xin-Yi Chu1  Tian Tian1  Peng-Fei Dong1  Bai-Xue Chen1  Hong-Yu Zhang1  | |
[1] Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University | |
关键词: cDNA display; c-Fos/c-Jun; evolution; proximity ligation; transcription factor; | |
DOI : 10.1002/1873-3468.13388 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Transcriptional regulation involves a series of sophisticated protein–protein and protein–DNA interactions (PPI and PDI). Some transcriptional complexes, such as c-Fos/c-Jun and their binding DNA fragments, have been conserved over the past one billion years. Considering the thermodynamic principle for transcriptional complex formation, we hypothesized that the cFos/c-Jun complex may represent a thermodynamic summit in the evolutionary space. To test this, we invented a new method, termed One-Pot-seq, which combines cDNA display and proximity ligation to analyse PPI/PDI complexes simultaneously. We found that the wild-type c-Fos/c-Jun complex is indeed the most thermodynamically stable relative to various mutants of cFos/c-Jun and binding DNA fragments. Our method also provides a universal approach to detect transcriptional complexes and explore transcriptional regulation mechanisms.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO202105310000126ZK.pdf | 1342KB | download |