Circular RNA SIPA1L1 regulates osteoblastic differentiation of stem cells from apical papilla via miR-204-5p/ALPL pathway

Stem Cell Research & Therapy

Xingyun Ge¹  Minxia Bian²  Zhou Zhou²  Yuzhi Li²  Jinhua Yu²  Xiao Wu²  Tong Xiao² 

[1] Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical University, 136 Hanzhong Road, 210029, Nanjing, Jiangsu, China;Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical University, 136 Hanzhong Road, 210029, Nanjing, Jiangsu, China;Endodontic Department, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, China;

关键词: circRNAs;    miR-204-5p;    ALPL;    SCAPs;    Differentiation;   

DOI  :  10.1186/s13287-020-01970-7

来源: Springer

【 摘 要 】

BackgroundOsteogenesis is a complex biological process which requires the coordination of multiple molecular mechanisms. This research aimed to explore the biological role and underlying regulatory mechanism of circSIPA1L1 during the osteogenic differentiation of stem cells from apical papilla (SCAPs).MethodsEdU retention assay, flow cytometry assay, and CCK-8 assay were used to evaluate the proliferation capacity of SCAPs. Western blot assay, alkaline phosphatase (ALP), and alizarin red staining (ARS) were conducted to investigate the biological roles of circSIPA1L1 and miR-204-5p. Fluorescence in situ hybridization was applied for circSIPA1L1 localization. Dual-luciferase reporter assay was performed to prove the interaction of circSIPA1L1 and miR-204-5p.ResultsCircSIPA1L1 had no significant effect on the proliferative capacity of SCAPs. CircSIPA1L1 promotes osteogenic differentiation of SCAPs by serving as a miRNA sponge for miR-204-5p. Either knockdown of circSIPA1L1 or overexpression of miR-204-5p significantly suppresses osteogenic differentiation of SCAPs.ConclusionsCircSIPA1L1 upregulates ALPL through targeting miR-204-5p and promotes the osteogenic differentiation of SCAPs.

【 授权许可】

CC BY