Combination of Dental Capping Agents With LowLevel Laser Therapy Increases the Cell Viability Percent of Stem Cells From Apical Papilla (SCAPs)

Journal of Lasers in Medical Sciences

article

Jaber Zafari¹  Hamed Karkehabadi²  Forough Nikzad²  Sogand Esmailnasab²  Zahra Abbasi Javan²  Fatemeh Javani Jouni³ 

[1] Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences;Department of Endodontics, Dental School, Hamadan University of Medical Science;Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University

关键词: MTA;    CEM;    Emdogain;    Low-level laser therapy (LLLT);    SCAPs;   

DOI  :  10.34172/jlms.2022.58

学科分类：基础医学

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Introduction: Stem cells from apical papillae (SCAPs) are adult stem cells capable of differentiating into multiple cell lineages. Dental pulp capping materials promote the differentiation of stem cells of the apical papilla (SCAPs). Higher proliferation and cell viability of stem cells have been reported upon exposure to low-level laser therapy (LLLT). However, there is limited evidence on the combinational effects of dental pulp capping materials and low-level laser radiation. In this study, the effects of dental dressing materials combined with laser on the SCAPs viability will assess. Methods: SCAPs isolation was performed from two immature third molar teeth through collagenase type I enzymatic activity. Isolated stem cells were then cultured with DMEM and α-MEM media enriched with 15% and 10% FBS, respectively. After reaching 70-80% confluency, cells were seeded in a 96-well plate. Cell viability percent was assessed using MTT assay after treatment by MTA, EMD, CEM, and LLLT alone and in combination for 24, 48, and 168 h. Results: A combination of MTA, CEM, EMD, and LLLT could result in significantly increased SCAPs viability as compared with other treatment groups. Increased levels of SCAPs proliferation and viability were observed in groups treated with the combination of MTA and CEM with EMD. SCAPs viability percent in all defined times was reduced with MTA and CEM treatment. Conclusion: LLLT is a stimulator of SCAPs cell viability percent and differentiation rate when applied with dental capping agents such as MTA, EMD, and CEM, which is a therapeutic option for stem cell-based therapy.

【 授权许可】

CC BY