HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis

Reproductive Biology and Endocrinology

Xiangjun Chen¹  Bo Deng¹  Hang Qi²  Wei Xia²  Guiling Liang²  Chenfeng Zhu²  Xiaoya Zhao²  Qian Zhu²  Jian Zhang²  Xiaoqing He²  Juan Li³ 

[1] Department of Neurology, Huashan Hospital and Institute of Neurology, Fudan University, No. 12 Wulumuqi Zhong Road, 200040, Shanghai, China;Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, No. 910, Hengshan Rd, 200030, Shanghai, China;Department of Pathology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 200030, Shanghai, China;

关键词: Ovarian teratoma;    Anti-N-methyl-D-aspartate receptor encephalitis;    HLA-A;    HLA-DRB1;    Autoantibodies;   

DOI  :  10.1186/s12958-020-00661-5

来源: Springer

【 摘 要 】

BackgroundOvarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis (NMDAR-E) is a severe autoimmune neurological disorder, and the influence of teratoma-induced autoantibodies on the pathogenesis remains unclear.MethodsOvarian teratoma tissues were collected from teratoma patients with and without NMDAR-E. Proteins were extracted and then analyzed using iTRAQ-coupled LC–MS/MS, which was followed by bioinformatics analysis. Candidate proteins were verified by Western blotting and immunohistochemistry.ResultsIn total, 36 differentially expressed proteins (DEPs) were identified between the control group and NMDAR-E group, and the bioinformatics analysis revealed that the DEPs were mainly involved in immune-related pathways, especially HLA-A and HLA-DRB1. The western blotting results for HLA-A and HLA-DRB1 were consistent with the results of the iTRAQ analysis. Additionally, the immunohistochemical data revealed that the aggregation of HLA-A (+) and HLA-DRB1 (+) cells was more apparent in the teratoma tissues of NMDAR-E patients compared with that in the tissues of controls.ConclusionOur investigation indicated that HLA-A and HLA-DRB1 might be involved in mediating ovarian teratoma-associated NMDAR-E. These findings provide new insights into the pathophysiological mechanisms and provide information for the functional exploration of proteins in the future.

【 授权许可】

CC BY