Typing class I HLA-A gene using a nested PCR-RFLP procedure

Brazilian Journal of Medical and Biological Research

E.c. Castelli²  D.s. Gil²  L.c.s. Veiga¹  J.l.v. De Camargo² 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Departamento de PatologiaBotucatu SP ,Brasil

关键词: Human leukocyte antigen-A;    HLA-A;    Typing;    RFLP;    Polymorphism;    Major histocompatibility complex;   

DOI  :  10.1590/S0100-879X2005000600004

来源: SciELO

【 摘 要 】

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.

【 授权许可】

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