Longitudinal assessment and stability of long non-coding RNA gene expression profiles measured in human peripheral whole blood collected into PAXgene blood RNA tubes

BMC Research Notes

Guzel I. Shaginurova¹  Lukasz S. Wylezinski²  Charles F. Spurlock III² 

[1] IQuity, Inc, 111, 10th Avenue South, Suite 100, 37203, Nashville, TN, USA;IQuity, Inc, 111, 10th Avenue South, Suite 100, 37203, Nashville, TN, USA;Decode Health, Inc, 209 10th Avenue South, Suite 404, Nashville, TN, USA;Department of Medicine, Vanderbilt University School of Medicine, 1161 21st Avenue South, Medical Center North T3113, Nashville, TN, USA;

关键词: Long non-coding RNA;    Multiple sclerosis;    Messenger RNA;    PAXgene blood RNA tube;    Quantitative real time PCR;    Storage;   

DOI  :  10.1186/s13104-020-05360-3

来源: Springer

【 摘 要 】

ObjectiveLong non-coding RNAs (lncRNAs) are emerging as novel biomarkers for a variety of chronic conditions including autoimmune disease. PAXgene Blood RNA tubes are routinely used in clinical research and molecular diagnostic development to capture RNA profiles in peripheral whole blood. While the stability of mRNA expression profiles captured using PAXgene tubes has been documented previously, no previous work has determined the stability of lncRNA expression profiles observed in PAXgene tubes stored at − 80 °C. Here we sought to determine the effects on lncRNA expression profiles following − 80 °C storage of total RNA templates, cDNA synthesized using fresh or frozen total RNA template, and the impact of freeze–thaw cycles on both total RNA and cDNA obtained from PAXgene tubes.ResultsWe find that storage of whole blood in PAXgene tubes, total RNA and cDNA for up to 1 year at − 80 °C or up to ten total RNA or cDNA freeze–thaw cycles do not significantly alter lncRNA expression profiles compared to baseline. As monthly expression profiles were determined, some month to month lncRNA expression variability was observed. However, all monthly observations fell within the 95% confidence interval calculated at baseline.

【 授权许可】

CC BY