Genome-wide identification of the restorer-of-fertility-like (RFL) gene family in Brassica napus and expression analysis in Shaan2A cytoplasmic male sterility

BMC Genomics

Jianjie He¹  Huaixin Li¹  Luyun Ning¹  Kang Chen¹  Hongbo Chao¹  Maoteng Li²  Dianrong Li³  Hao Wang³  Yonghong Li³ 

[1] Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, China;Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, China;Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, 438000, Huanggang, China;Hybrid Rape Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement, 712100, Yangling, China;

关键词: CMS;    Rf;    RFL;    PPR;    RNA-sequencing;   

DOI  :  10.1186/s12864-020-07163-z

来源: Springer

【 摘 要 】

BackgroundCytoplasmic male sterility (CMS) is very important in hybrid breeding. The restorer-of-fertility (Rf) nuclear genes rescue the sterile phenotype. Most of the Rf genes encode pentatricopeptide repeat (PPR) proteins.ResultsWe investigated the restorer-of-fertility-like (RFL) gene family in Brassica napus. A total of 53 BnRFL genes were identified. While most of the BnRFL genes were distributed on 10 of the 19 chromosomes, gene clusters were identified on chromosomes A9 and C8. The number of PPR motifs in the BnRFL proteins varied from 2 to 19, and the majority of BnRFL proteins harbored more than 10 PPR motifs. An interaction network analysis was performed to predict the interacting partners of RFL proteins. Tissue-specific expression and RNA-seq analyses between the restorer line KC01 and the sterile line Shaan2A indicated that BnRFL1, BnRFL5, BnRFL6, BnRFL8, BnRFL11, BnRFL13 and BnRFL42 located in gene clusters on chromosomes A9 and C8 were highly expressed in KC01.ConclusionsIn the present study, identification and gene expression analysis of RFL gene family in the CMS system were conducted, and seven BnRFL genes were identified as candidates for the restorer genes in Shaan2A CMS. Taken together, this method might provide new insight into the study of Rf genes in other CMS systems.

【 授权许可】

CC BY