A comparative study of Candida species diversity among patients with oral squamous cell carcinoma and oral potentially malignant disorders

BMC Research Notes

Krishnan Mahalakshmi¹  Sankar Leena Sankari²  Venkatesan Naveen Kumar³ 

[1] Department of Microbiology, Research Lab for Oral-systemic Health, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, 600100, Chennai, Tamil Nadu, India;Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, 600100, Chennai, Tamil Nadu, India;Research Lab for Oral-systemic Health, Sree Balaji Dental College and Hospital/ImmuGenix Biosciences Pvt Ltd, No 16/2 Nattal Garden 1st Street, Perambur, 600011, Chennai, Tamil Nadu, India;

关键词: Candida;    Oral squamous cell carcinoma;    Oral potentially malignant disorder;    Polymerase chain reaction;    Restriction fragment length polymorphism;   

DOI  :  10.1186/s13104-020-05336-3

来源: Springer

【 摘 要 】

ObjectivesTo determine the prevalence of Candida species by PCR–RFLP method in the saliva of patients with oral squamous cell carcinoma (OSCC), oral potentially malignant disorders (OPMD) and healthy cohorts. Unstimulated saliva was collected from patients with OSCC (n = 97), OPMD (n = 200), and healthy controls (n = 200). Candida species were isolated using the standard protocol. The isolates were identified using phenotypic and genotypic methods. The odds/risk ratio was calculated using Pearson’s Chi-square test. The significance of Candidal carriage was calculated by independent T-test.ResultsOral Candidal carriage was 72.2%, 58% and 20.5% among patients with OSCC, OPMD, and healthy controls respectively. The oral Candidal carriage in OSCC and OPMD was highly significant (p = 0.0001). Non albicans Candida predominated over Candida albicans. Candida species were diverse among the study groups with a predominance of Candida krusei, Candida tropicalis, and Pichia anomala formerly Candida pelliculosa. P. anomala occurrence outnumbered in health. The odds/risk ratio for OSCC and OPMD were 4.25/11.87 and 3.52/6.99 respectively. A high prevalence of non albicans Candida was observed both in all the three groups (OSCC, OPMD and healthy controls). High odds and risk ratio associates Candida species to OSCC and OPMD. Candida famata may be associated with OSCC and OPMD.

【 授权许可】

CC BY