Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Revista da Sociedade Brasileira de Medicina Tropical

Paula Cristhina Niz Xavier¹  Marilene Rodrigues Chang¹  Claudete Rodrigues Paula¹  Flavia Emi Matsumoto¹  Marise Dutra Asensi¹  Maria De Fatima Cepa Matos¹  Renato Andreotti¹ 

关键词: Candida;    Polymerase chain reaction;    Random amplification of polymorphic DNA;   

DOI  :  10.1590/0037-8682-1718-2013

来源: SciELO

【 摘 要 】

IntroductionThe aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures.MethodsRandom amplification of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, state of Mato Grosso do Sul, Brazil, from 1998-2000.Results The most frequently isolated species was Candida albicans (45.8%). DNA amplification from genomic yeast isolates indicated a genetic similarity of over 90%.ConclusionsThe RAPD profiles obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.

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