Comparative transcriptome study of switchgrass (Panicum virgatum L.) homologous autopolyploid and its parental amphidiploid responding to consistent drought stress

Biotechnology for Biofuels

Xinquan Zhang¹  Min Sun¹  Xiaoshan Wang¹  Linkai Huang¹  Jing Chen¹  Guangyan Feng¹  Bingchao Wu¹  Haidong Yan²  Peilin Chen³ 

[1] Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, 611130, Chengdu, China;Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, 611130, Chengdu, China;Department of Horticulture, Virginia Tech, 24061, Blacksburg, VA, USA;Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, 611130, Chengdu, China;Institute for Biology, Plant Cell and Molecular Biology, Humboldt-Universität zu Berlin, 10115, Berlin, Germany;

关键词: Panicum virgatum;    Whole genome duplication;    Drought response;    MicroRNA;    Transcriptome;    WGCNA;   

DOI  :  10.1186/s13068-020-01810-z

来源: Springer

【 摘 要 】

BackgroundNewly formed polyploids may experience short-term adaptative changes in their genome that may enhance the resistance of plants to stress. Considering the increasingly serious effects of drought on biofuel plants, whole genome duplication (WGD) may be an efficient way to proceed with drought resistant breeding. However, the molecular mechanism of drought response before/after WGD remains largely unclear.ResultWe found that autoploid switchgrass (Panicum virgatum L.) 8X Alamo had higher drought tolerance than its parent amphidiploid 4X Alamo using physiological tests. RNA and microRNA sequencing at different time points during drought were then conducted on 8X Alamo and 4X Alamo switchgrass. The specific differentially expressed transcripts (DETs) that related to drought stress (DS) in 8X Alamo were enriched in ribonucleoside and ribonucleotide binding, while the drought-related DETs in 4X Alamo were enriched in structural molecule activity. Ploidy-related DETs were primarily associated with signal transduction mechanisms. Weighted gene co-expression network analysis (WGCNA) detected three significant DS-related modules, and their DETs were primarily enriched in biosynthesis process and photosynthesis. A total of 26 differentially expressed microRNAs (DEmiRs) were detected, and among them, sbi-microRNA 399b was only expressed in 8X Alamo. The targets of microRNAs that were responded to polyploidization and drought stress all contained cytochrome P450 and superoxide dismutase genes.ConclusionsThis study explored the drought response of 8X and 4X Alamo switchgrass on both physiological and transcriptional levels, and provided experimental and sequencing data basis for a short-term adaptability study and drought-resistant biofuel plant breeding.

【 授权许可】

CC BY