Alterations in bacterial communities, SCFA and biomarkers in an elderly HIV-positive and HIV-negative population in western Mexico

BMC Infectious Diseases

Julio A. Díaz-Ramos¹  Alvaro López-Iñiguez²  Moises Ramos-Solano²  RA Soria-Rodriguez²  Monserrat Alvarez-Zavala²  Karina Sánchez-Reyes²  Natali Vega-Magaña²  Luz A. González-Hernández³  Pedro Martínez-Ayala³  Jaime F. Andrade-Villanueva³  Mariana del Rocio Ruiz-Briseño⁴ 

[1] Geriatric Department, Antiguo Hospital Civil de Guadalajara “Fray Antonio Alcalde”, Guadalajara, Jalisco, Mexico;HIV and Immunodeficiencies Research Institute, Clinical Medicine Department, CUCS-University of Guadalajara, Guadalajara, Jalisco, Mexico;HIV and Immunodeficiencies Research Institute, Clinical Medicine Department, CUCS-University of Guadalajara, Guadalajara, Jalisco, Mexico;HIV Unit Department, University Hospital “Fray Antonio Alcalde”, University of Guadalajara, Guadalajara, Jalisco, Mexico;HIV and Immunodeficiencies Research Institute, Clinical Medicine Department, CUCS-University of Guadalajara, Guadalajara, Jalisco, Mexico;Molecular Biology in Medicine Ph. D. program, CUCS-University of Guadalajara, Guadalajara, Mexico;

关键词: HIV-infection;    Stool microbiota;    Elderly population;    Biomarkers;    SCFAs;   

DOI  :  10.1186/s12879-019-3867-9

来源: Springer

【 摘 要 】

BackgroundThe study of stool microbiota has taken great relevance in the last years, given its role in the maintenance of the intestinal metabolic, physiological, and immunological homeostasis, as well as, its effect over HIV biomarkers levels such as CD4/CD8 ratio, high sensitivity C-Reactive Protein (hs-CRP), related to poor outcomes (rapid progression to AIDS). Several efforts have been made to characterize the gut microbiome. In HIV infection, most of the studies report the presence of a dysbiotic pattern; however, few of them have made an approach in elderly HIV-positive subjects despite the fact that nowadays this subgroup is rising. In this study, we compared the composition of faecal microbiota, Short Chain Fatty Acids (SCFAs), and systemic biomarkers between elderly HIV-positive and HIV-negative subjects.MethodsA cross-sectional study with 18 HIV-negative controls and 20 HIV-positive patients. The quantification of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, Lactobacillus, Enterobacteriaceae, Bifidobacterium, Escherichia coli, Clostridium leptum, Clostridium coccoides was performed in faecal samples by qPCR. The analysis was performed by calculating the ΔCq of each microorganism using 16S rDNA as a reference gene. Faecal SCFAs were measured by HPLC. The hs-CRP and sCD14 were performed by ELISA.ResultsAn increase in the Firmicutes/Bacteroidetes ratio, coupled with a significant increase in the proteobacteria phylum was detected in HIV-positive subjects. In contrast, a decrease in the Clostridium leptum group was observed. Nevertheless, these elderly HIV-positive patients showed higher levels of total SCFAs mainly by an augmented propionic acid values, compared to HIV-negative subjects. Whereas high levels of hs-CRP were positively correlated with sCD14 in the HIV-positive group.ConclusionsAlterations in bacterial communities reveals a dysbiotic state related to an unbalance of faecal SCFAs. Therefore, these intestinal conditions might drive an increase of poor prognostic biomarkers in elderly HIV-positive subjects.

【 授权许可】

CC BY