Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

Journal of Orthopaedic Surgery and Research

Ryo Tazawa¹  Terumasa Matsuura¹  Tadashi Kawamura¹  Hiroaki Minehara¹  Kyoko Muneshige¹  Gen Inoue¹  Sho Inoue¹  Masashi Takaso¹  Kentaro Uchida²  Hiroyuki Sekiguchi³ 

[1] Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara City, Kanagawa, Japan;Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara City, Kanagawa, Japan;Shonan University of Medical Sciences Research Institute, Nishikubo 500, 253-0083, Chigasaki City, Kanagawa, Japan;Shonan University of Medical Sciences Research Institute, Nishikubo 500, 253-0083, Chigasaki City, Kanagawa, Japan;

关键词: Collagen-like peptides;    Poly(POG)n;    Bone defect;    New bone formation;    Bone morphogenetic protein-2;   

DOI  :  10.1186/s13018-020-01977-z

来源: Springer

【 摘 要 】

BackgroundDelivery of bone morphogenetic protein-2 (BMP-2) via animal-derived absorbable collagen materials is used for the treatment of large bone defects. However, the administration of bovine proteins to humans is associated with the risk of zoonotic complications. We therefore examined the effect of combining BMP-2 with collagen-like peptides, poly(POG)n, in a critical-sized bone defect mouse model.MethodsA 2-mm critical-sized bone defect was created in the femur of 9-week-old male C57/BL6J mice. Mice were randomly allocated into one of four treatment groups (n = 6 each): control (no treatment), poly(POG)n only, 0.2 μg, or 2.0 μg BMP-2 with poly(POG)n. New bone formation was monitored using soft X-ray radiographs, and bone formation at the bone defect site was examined using micro-computed tomography and histological examination at 4 weeks after surgery.ResultsAdministration of 2.0 μg of BMP-2 with poly(POG)n promoted new bone formation and resulted in greater bone volume and bone mineral content than that observed in the control group and successfully achieved consolidation. In contrast, bone formation in all other groups was scarce.ConclusionsOur findings suggest the potential of BMP-2 with poly(POG)n as a material, free from animal-derived collagen, for the treatment of large bone defects.

【 授权许可】

CC BY