Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso

BMC Pediatrics

Nathalie K. Guessennd¹  Ali Konaté²  Wendpoulomdé A. D. Kaboré²  Nicolas Barro²  Alfred S. Traoré²  Assèta Kagambèga³  Oumar Traoré⁴  René Dembélé⁴  Issiaka Soulama⁵  Amy Gassama-Sow⁶  Awa Aidara-Kane⁶ 

[1]Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials, Pasteur Institute of Abidjan, 01, BP 490, Abidjan 01, Ivory Coast

[2]Laboratory of Bacteriology-Virology, Unit of Training and Research of Medical Sciences, University Felix Houphouet BOIGNY, 01, BP V34, Abidjan 01, Ivory Coast

[3]Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso

[4]Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso

[5]Institute of Sciences, 01, BP 1757, Ouagadougou 01, Burkina Faso

[6]Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso

[7]Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso

[8]National Centre for Research and Training on Malaria, 01, BP 2208, Ouagadougou 01, Burkina Faso

[9]Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal

关键词: Antibiotics;    Resistance genes;    qnr;    bla;    bla;   

DOI  :  10.1186/s12887-020-02342-z

来源: Springer

【 摘 要 】

BackgroundThe emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.MethodsMultiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.ResultsThe Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX−M gene and the qnrB gene simultaneously.ConclusionsThis study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.

【 授权许可】

CC BY