Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1

Cancer Cell International

Qingling Zheng¹  Yan Zheng²  Wei Fang²  Jin Zhang³  Donghua Gu³  Yanxiang Liu³  Xin Dai³ 

[1] Department of Obstetrics and Gynecology, School of Medicine and Nursing Sciences, Huzhou University, Huzhou Central Hospital, 313000, Huzhou, People’s Republic of China;Department of Pathology, Huzhou Central Hospital, 313000, Huzhou, People’s Republic of China;Department of Pathology, The Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University, No. 1, Lijiang Road, Huqiu District, 215153, Suzhou, Jiangsu, People’s Republic of China;

关键词: Ovarian cancer;    microRNA-367;    Lysophosphatidic acid receptor-1;    Proliferation;    Invasion;    Angiogenesis;   

DOI  :  10.1186/s12935-020-01551-x

来源: Springer

【 摘 要 】

BackgroundCompelling evidences reported the role of microRNAs (miRNAs) in ovarian cancer. However, little was known regarding the molecular mechanism of miR-367 in ovarian cancer. This study intended to investigate the role and regulatory mechanism of miR-367 in ovarian cancer involving lysophosphatidic acid receptor-1 (LPA1).MethodsPotentially regulatory miRNAs in ovarian cancer were obtained from bioinformatics analysis. RT-qPCR was used to detect miR-367 expression in both ovarian cancer tissues and relevant adjacent normal tissues. Relationship between miR-367 and LPA1 was predicted by miRNA database and further verified using dual luciferase reporter gene assay and RIP. EdU and Transwell assay were used to measure the proliferation and invasion ability of cells. Moreover, tube formation and chick chorioallantois membrane (CAM) assay were performed to determine angiogenesis of human umbilical vein endothelial cells (HUVECs). Finally, the roles of LPA1 in tumor growth was also studied using nude mice xenograft assay.ResultsHigh expression of LPA1 and low expression of miR-367 were observed in ovarian cancer tissues and cells. Overexpressed miR-367 downregulated LPA1 expression to inhibit proliferation, invasion, and angiogenesis of cancer cells. Low expression of LPA1 suppressed tumor formation and repressed angiogenesis in ovarian in vivo.ConclusionAll in all, overexpression of miR-367 downregulated LPA1 expression to inhibit ovarian cancer progression, which provided a target for the cancer treatment.

【 授权许可】

CC BY