iTRAQ-based comparative proteomic analysis of differences in the protein profiles of stems and leaves from two alfalfa genotypes

BMC Plant Biology

Qingchuan Yang¹  Mingna Li¹  Wenwen Liu¹  Ruicai Long¹  Junmei Kang¹  Zhen Wang¹  Fan Zhang¹  Jie Yu¹  Jiaju Zhang¹  Hao Sun² 

[1] Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, Guangdong, China;

关键词: Alfalfa;    Proteomics;    Stem;    Leaf;    Lignin synthesis;    Photosynthesis;   

DOI  :  10.1186/s12870-020-02671-2

来源: Springer

【 摘 要 】

BackgroundTo explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype alfalfa, with thick stems and large leaves.ResultsBased on fold-change thresholds of > 1.20 or < 0.83 (p < 0.05), a large number of proteins were identified as being differentially enriched between the M and F genotypes: 249 downregulated and 139 upregulated in stems and 164 downregulated and 134 upregulated in leaves. The differentially enriched proteins in stems were mainly involved in amino acid biosynthesis, phenylpropanoid biosynthesis, carbon fixation, and phenylalanine metabolism. The differentially enriched proteins in leaves were mainly involved in porphyrin and chlorophyll metabolism, phenylpropanoid biosynthesis, starch and sucrose metabolism, and carbon fixation in photosynthetic organisms. Six differentially enriched proteins were mapped onto the porphyrin and chlorophyll metabolism pathway in leaves of the M genotype, including five upregulated proteins involved in chlorophyll biosynthesis and one downregulated protein involved in chlorophyll degradation. Eleven differentially enriched proteins were mapped onto the phenylpropanoid pathway in stems of the M genotype, including two upregulated proteins and nine downregulated proteins.ConclusionEnhanced chlorophyll synthesis and decreased lignin synthesis provided a reasonable explanation for the larger leaves and lower levels of stem lignification in M genotype alfalfa. This proteomic study aimed to classify the functions of differentially enriched proteins and to provide information on the molecular regulatory networks involved in stem and leaf development.

【 授权许可】

CC BY