Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation

Nano-Micro Letters

Bart Vandekerckhove¹  Stijn De Munter¹  Glenn Goetgeluk¹  Lien Van Hoecke²  Ranhua Xiong³  Juan C. Fraire³  Toon Brans⁴  Stephan Stremersch⁴  Jelter Van Hoeck⁴  Stefaan De Smedt⁴  Rein Verbeke⁴  Koen Raemdonck⁴  Kevin Braeckmans⁴  Laurens Raes⁴  Xavier Saelens⁵ 

[1] Cancer Research Institute Ghent (CRIG), 9000, Ghent, Belgium;Department of Diagnostic Sciences, Ghent University, 9000, Ghent, Belgium;Cancer Research Institute Ghent (CRIG), 9000, Ghent, Belgium;VIB-UGent Center for Medical Biotechnology, 9052, Ghent, Belgium;Department of Biomedical Molecular Biology, Ghent University, 9000, Ghent, Belgium;Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, 9000, Ghent, Belgium;Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, 9000, Ghent, Belgium;Cancer Research Institute Ghent (CRIG), 9000, Ghent, Belgium;VIB-UGent Center for Medical Biotechnology, 9052, Ghent, Belgium;Department of Biochemistry and Microbiology, Ghent University, 9000, Ghent, Belgium;

关键词: Transfection;    mRNA;    Photoporation;    Optoporation;    Gold nanoparticles;    Vapor nanobubbles;   

DOI  :  10.1007/s40820-020-00523-0

来源: Springer

【 摘 要 】

tsVapor nanobubble (VNB) photoporation represents a promising physical technique for mRNA transfection of adherent and suspension cells.A multitude of parameters related to the VNB photoporation procedure were optimized to enable efficient mRNA transfection.VNB photoporation was found to yield five times more living, transfected Jurkat T cells as compared to electroporation, i.e., currently the standard nonviral transfection technique for T cells.

【 授权许可】

CC BY