Tumor suppressive function of Matrin 3 in the basal-like breast cancer

Biological Research

Yongseok Kwon¹  Li Ma²  Jaehyuk Yang³  Seung Jun Lee³  Jongchan Kim³ 

[1] Department of Chemistry, Sogang University, Seoul, Republic of Korea;Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Life Sciences, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, Republic of Korea;

关键词: MATR3;    Basal-like breast cancer;    Triple-negative breast cancer;    Apoptosis;    Tumor suppressor;    Epithelial-mesenchymal transition;    YAP/TAZ;    Biomarker;   

DOI  :  10.1186/s40659-020-00310-6

来源: Springer

【 摘 要 】

BackgroundBasal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whose cancer-relevance has not been characterized.MethodsWe analyzed in vitro tumorigenecity by cell proliferation and soft agar colony formation assays, apoptotic cell death by flow cytometry and Poly (ADP-ribose) polymerase (PARP) cleavage, epithelial-mesenchymal transition (EMT) by checking specific EMT markers with real-time quantitative PCR and in vitro migration and invasion by Boyden Chamber assays. To elucidate the underlying mechanism by which MATR3 functions as a tumor suppressor, we performed Tandem affinity purification followed by mass spectrometry (TAP-MS) and pathway analysis. We also scrutinized MATR3 expression levels in the different subtypes of human breast cancer and the correlation between MATR3 expression and patient survival by bioinformatic analyses of publicly available transcriptome datasets.ResultsMATR3 suppressed in vitro tumorigenecity, promoted apoptotic cell death and inhibited EMT, migration, and invasion in BLBC/TNBC cells. Various proteins regulating apoptosis were identified as MATR3-binding proteins, and YAP/TAZ pathway was suppressed by MATR3. MATR3 expression was inversely correlated with the aggressive and metastatic nature of breast cancer. Moreover, high expression levels of MATR3 were associated with a good prognosis of breast cancer patients.ConclusionsOur data demonstrate that MATR3 functions as a putative tumor suppressor in BLBC/TNBC cells. Also, MATR3 potentially plays a role as a biomarker in predicting chemotherapy-sensitivity and patient survival in breast cancer patients.

【 授权许可】

CC BY