Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

AMB Express

Mariana B. Méndez¹  Jorge A. Trelles¹  Cintia W. Rivero¹ 

[1] Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD, Bernal, Argentina;National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB, Caba, Argentina;

关键词: Biocatalysis;    Nucleoside 2′-deoxyribosyltransferase;    Bentonite;    Immobilization;   

DOI  :  10.1186/s13568-020-01109-0

来源: Springer

【 摘 要 】

A novel IDA-LaNDT derivative was able to reach the highest productivity in the biosynthesis of a well-known antitumoral agent called decitabine. However, the combination of two simple and inexpensive techniques such as ionic absorption and gel entrapment with the incorporation of a bionanocomposite such as bentonite significantly improved the stability of this biocatalyst. These modifications allowed the enhancement of storage stability (for at least 18 months), reusability (400 h of successive batches without significant loss of its initial activity), and thermal and solvent stability with respect to the non-entrapped derivative. Moreover, reaction conditions were optimized by increasing the solubility of 5-aza by dilution with dimethylsulfoxide. Therefore, a scale-up of the bioprocess was assayed using the developed biocatalyst, obtaining 221 mg/L·h of DAC. Finally, green parameters were calculated using the nanostabilized biocatalyst, whose results indicated that it was able to biosynthesize DAC by a smooth, cheap, and environmentally friendly methodology.

【 授权许可】

CC BY