期刊论文详细信息
AMB Express | |
Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites | |
Mariana B. Méndez1  Jorge A. Trelles1  Cintia W. Rivero1  | |
[1]Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD, Bernal, Argentina | |
[2]National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB, Caba, Argentina | |
关键词: Biocatalysis; Nucleoside 2′-deoxyribosyltransferase; Bentonite; Immobilization; | |
DOI : 10.1186/s13568-020-01109-0 | |
来源: Springer | |
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【 摘 要 】
A novel IDA-LaNDT derivative was able to reach the highest productivity in the biosynthesis of a well-known antitumoral agent called decitabine. However, the combination of two simple and inexpensive techniques such as ionic absorption and gel entrapment with the incorporation of a bionanocomposite such as bentonite significantly improved the stability of this biocatalyst. These modifications allowed the enhancement of storage stability (for at least 18 months), reusability (400 h of successive batches without significant loss of its initial activity), and thermal and solvent stability with respect to the non-entrapped derivative. Moreover, reaction conditions were optimized by increasing the solubility of 5-aza by dilution with dimethylsulfoxide. Therefore, a scale-up of the bioprocess was assayed using the developed biocatalyst, obtaining 221 mg/L·h of DAC. Finally, green parameters were calculated using the nanostabilized biocatalyst, whose results indicated that it was able to biosynthesize DAC by a smooth, cheap, and environmentally friendly methodology.【 授权许可】
CC BY
【 预 览 】
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RO202104246211692ZK.pdf | 1677KB | ![]() |