Immobilization of Lipase on Single Walled Carbon Nanotubes in Ionic Liquid

Bulletin of the Korean chemical society

Han Ki Lee¹  Cheol Jin Lee¹  Jae Kwan Lee¹  Mahn Joo Kim¹ 

关键词: Lipase;    Single walled carbon nanotube;    Immobilization;    Ionic liquid;    Biocatalysis;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

【 授权许可】

Unknown