Bioresources and Bioprocessing | |
The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture | |
Paul Jensen1  Peter James Strong2  Bronwyn Laycock3  Paul Andrew Lant3  Syarifah Nuraqmar Syed Mahamud3  Steven Pratt3  Pawarisa Luangthongkam4  Gene Tyson5  Paul Evans5  | |
[1] Advanced Water Management Centre, The University of Queensland, 4072, Brisbane, QLD, Australia;Queensland University of Technology, 2 George Street, 4000, Brisbane, QLD, Australia;School of Chemical Engineering, The University of Queensland, 4072, St. Lucia, Brisbane, QLD, Australia;School of Chemical Engineering, The University of Queensland, 4072, St. Lucia, Brisbane, QLD, Australia;Queensland University of Technology, 2 George Street, 4000, Brisbane, QLD, Australia;The Australian Centre for Ecogenomics, The University of Queensland, 4072, Brisbane, QLD, Australia; | |
关键词: Methane; Methanotrophs; Polyhydroxyalkanoates; 3-Hydroxybutyrate; 3-Hydroxyvalerate; | |
DOI : 10.1186/s40643-019-0285-1 | |
来源: Springer | |
【 摘 要 】
A methanotrophic community was enriched in a semi-continuous reactor under non-aseptic conditions with methane and ammonia as carbon and nitrogen source. After a year of operation, Methylosinus sp., accounted for 80% relative abundance of the total sequences identified from potential polyhydroxyalkanoates (PHAs) producers, dominated the methane-fed enrichment. Prior to induction of PHA accumulation, cells harvested from the parent reactor contained low level of PHA at 4.0 ± 0.3 wt%. The cells were later incubated in the absence of ammonia with various combinations of methane, propionic acid, and valeric acid to induce biosynthesis of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Previous studies reported that methanotrophic utilization of odd-chain fatty acids for the production of PHAs requires reducing power from methane oxidation. However, our findings demonstrated that the PHB-containing methanotrophic enrichment does not require methane availability to generate 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV)—when odd-chain fatty acids are presented. The enrichment yielded up to 14 wt% PHA with various mole fractions of 3HV monomer depending on the availability of methane and odd-fatty acids. Overall, the addition of valeric acid resulted in a higher PHA content and a higher 3HV fraction. The highest 3HV fraction (up to 65 mol%) was obtained from the methane–valeric acid experiment, which is higher than those previously reported for PHA-producing methanotrophic mixed microbial cultures.
【 授权许可】
CC BY
【 预 览 】
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RO202104241417295ZK.pdf | 1970KB | download |