Intramuscular 3-Hydroxybutyrate Levels after 60Tetani/Min Contraction in Diabetic and Non-Diabetic Rats

Endocrine Journal

NAOYA NAKAI²  YOSHIHARU SHIMOMURA¹  ISAO OHSAWA²  JUICHI SATO²  YOSHIHARU OSHIDA²  YUZO SATO² 

[1] Nagoya Institute of Technology;Research Center of Health, Physical Fitness and Sports, Nagoya University

关键词: 3-Hydroxybutyrate;    Microdialysis;    Muscle contraction;    Diabetes;   

DOI  :  10.1507/endocrj.45.363

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(21)Cited-By(1)Post-exercise hyperketonemia in poorly-controlled diabetic patients is a well recognized phenomenon, but because studies concerning changes in ketone body metabolism in muscle during and/or after exercise are scarce, we measured the intramuscular 3-hydroxybutyrate (3-OHBA) and lactate concentrations in 12 diabetic (streptozotocin-induced; 60mg/kg, ip) and 13 non-diabetic control rats before and after a 1-h muscle contraction. One thigh tetanic contraction was elicited at a tetanic frequency of 60tetani/min and the other thigh was kept resting. We used a microdialysis technique in both quadriceps muscles, vastus lateralis, and the right jugular vein. Blood flow in both femoral muscles was also monitored before and after contraction. Dialysate 3-OHBA and lactate levels in contracting and non-contracting muscles and in blood showed a significant increase after contraction (P<0.05) in diabetic rats. In control rats there was no significant change in dialysate 3-OHBA or the lactate concentration in contracting and non-contracting muscles or in blood before and after contraction. A significant increase in contracting muscle blood flow was observed only for the first 5 min after contraction in diabetic and control rats. These results suggest that 3-OHBA uptake in contracting muscle is reduced, and that this phenomenon may play a role in post-exercise ketosis in diabetes.

【 授权许可】

Unknown