Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

BMC Plant Biology

Li Xuan Ma¹  Yang Tian¹  Li Xiao²  Chou Fei Wu²  Zhongshan Zhang²  Xueting Zhong²  Zhan Qi Wang²  Li Qin Zhang³  Wei Fan⁴  Qi Ming Zhao⁴ 

[1] College of Food Science and Technology, Yunnan Agricultural University, 650201, Kunming, China;Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, 313000, Huzhou, China;Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, 313000, Huzhou, China;Huzhou central hospital, Huzhou University, 313000, Huzhou, China;State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, 650201, Kunming, China;

关键词: High-temperature stress;    Maca;    Molecular mechanism;    Stress response;    Tandem mass tag;   

DOI  :  10.1186/s12870-020-02645-4

来源: Springer

【 摘 要 】

BackgroundHigh-temperature stress (HTS) is one of the main environmental stresses that limit plant growth and crop production in agricultural systems. Maca (Lepidium meyenii) is an important high-altitude herbaceous plant adapted to a wide range of environmental stimuli such as cold, strong wind and UV-B exposure. However, it is an extremely HTS-sensitive plant species. Thus far, there is limited information about gene/protein regulation and signaling pathways related to the heat stress responses in maca. In this study, proteome profiles of maca seedlings exposed to HTS for 12 h were investigated using a tandem mass tag (TMT)-based proteomic approach.ResultsIn total, 6966 proteins were identified, of which 300 showed significant alterations in expression following HTS. Bioinformatics analyses indicated that protein processing in endoplasmic reticulum was the most significantly up-regulated metabolic pathway following HTS. Quantitative RT-PCR (qRT-PCR) analysis showed that the expression levels of 19 genes encoding proteins mapped to this pathway were significantly up-regulated under HTS. These results show that protein processing in the endoplasmic reticulum may play a crucial role in the responses of maca to HTS.ConclusionsOur proteomic data can be a good resource for functional proteomics of maca and our results may provide useful insights into the molecular response mechanisms underlying herbal plants to HTS.

【 授权许可】

CC BY