Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression

BMC Veterinary Research

Sabreen E. Fadl¹  Doaa H. Assar²  Ayman Atiba³  Hamed A. El-Serehy⁴  Mohamed M. Abdel-Daim⁵  Zizy I. Elbialy⁶  Ibrahim I. Al-Hawary⁶  Ahmed Elsheshtawy⁶  Aml Abdelnaby⁶ 

[1] Biochemistry Department, Faculty of Veterinary Medicine, Matrouh University, 51744, Matrouh, Egypt;Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el-Sheikh, Egypt;Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el-Sheikh, Egypt;Department of Zoology, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia;Department of Zoology, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia;Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 41522, Ismailia, Egypt;Fish processing and Biotechnology Department, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr el-Sheikh, Egypt;

关键词: Tilapia collagen;    Wound healing;    VEGF;    bFGF;    TGF-ß;    α-SMA;    IHC;    Histopathology;    Gene expression;   

DOI  :  10.1186/s12917-020-02566-2

来源: Springer

【 摘 要 】

BackgroundCollagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR.ResultsThe histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis.ConclusionsThe collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics.

【 授权许可】

CC BY