Overexpression of hepatocyte growth factor/scatter factor promotes vascularization and granulation tissue formation in vivo

FEBS Letters

Fukusato, Toshio³  Mori, Masatomo¹  Takagi, Hitoshi¹  Merlino, Glenn²  Toyoda, Mitsuo¹  Takayama, Hisashi¹  Otsuka, Toshiyuki¹  Horiguchi, Norio¹ 

[1] First Department of Internal Medicine, Gunma University School of Medicine, Showa-Machi 3-39-15, Maebashi 371-8511, Gunma, Japan;Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892-4255, USA;Division of Diagnostic Pathology, Gunma University School of Medicine, Showa-Machi 3-39-15, Maebashi 371-8511, Gunma, Japan

关键词: Hepatocyte growth factor/scatter factor;    Vascular endothelial growth factor;    Wound healing;    Angiogenesis;    Transgenic mouse;    bFGF;    basic fibroblast growth factor;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    H&E;    hematoxylin and eosin;    HGF/SF;    hepatocyte growth factor/scatter factor;    HPF;    high power field;    PECAM-1;    platelet endothelial cell adhesion molecule-1;    TGF;    transforming growth factor;    VEGF;    vascular endothelial growth factor;   

DOI  :  10.1016/S0014-5793(01)03126-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of hepatocyte growth factor/scatter factor (HGF/SF) during wound healing in the skin was investigated, using HGF/SF-overexpressing transgenic mouse model. Histological analysis of HGF/SF transgenic mouse excisional wound sites revealed increased granulation tissue with marked vascularization. Northern blot analysis demonstrated that, relative to control, vascular endothelial growth factor (VEGF) expression in transgenic skin was significantly higher at baseline and was robustly up-regulated during wound healing. Elevated levels of VEGF protein were detected immunohistochemically, predominantly in endothelial cells and fibroblasts within the granulation tissue of HGF/SF transgenic skin. Serum levels of VEGF were also elevated in HGF/SF transgenic mice. Thus, results from our study suggest that HGF/SF has a significant effect on vascularization and granulation tissue formation during wound healing in vivo, involving with induction of VEGF.

【 授权许可】

Unknown