Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus

Food Science and Technology (Campinas)

Micheline S. Ramella¹  Mariela A. Kroth¹  Caroline Tagliari¹  Ana Carolina M. Arisi¹ 

[1] ,Universidade Federal de Santa Catarina Departamento de Ciência e Tecnologia de Alimentos Florianópolis SC ,Brazil

关键词: fraud;    fish identification;    blackfin goosefish;    RAPD-PCR;    fraude;    identificação de peixes;    peixe sapo;    RAPD-PCR;   

DOI  :  10.1590/S0101-20612005000400017

来源: SciELO

【 摘 要 】

Lophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.

【 授权许可】

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