Acute stress-induced antinociception is cGMP-dependent but heme oxygenase-independent

Brazilian Journal of Medical and Biological Research

P.g. Carvalho-costa¹  L.g.s. Branco¹  C.r.a. Leite-panissi¹ 

关键词: Heme oxygenase;    Carbon monoxide;    cGMP;    Lateral cerebral ventricle;    Acute stress;    Antinociception;   

DOI  :  10.1590/1414-431X20143926

来源: SciELO

【 摘 要 】

Endogenous carbon monoxide (CO), which is produced by the enzyme heme oxygenase (HO), participates as a neuromodulator in physiological processes such as thermoregulation and nociception by stimulating the formation of 3′,5′-cyclic guanosine monophosphate (cGMP). In particular, the acute physical restraint-induced fever of rats can be blocked by inhibiting the enzyme HO. A previous study reported that the HO-CO-cGMP pathway plays a key phasic antinociceptive role in modulating noninflammatory acute pain. Thus, this study evaluated the involvement of the HO-CO-cGMP pathway in antinociception induced by acute stress in male Wistar rats (250-300 g; n=8/group) using the analgesia index (AI) in the tail flick test. The results showed that antinociception induced by acute stress was not dependent on the HO-CO-cGMP pathway, as neither treatment with the HO inhibitor ZnDBPG nor heme-lysinate altered the AI. However, antinociception was dependent on cGMP activity because pretreatment with the guanylate cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3-a] quinoxaline-1-one (ODQ) blocked the increase in the AI induced by acute stress.

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