Pressure-induced dissociation of casein micelles: size distribution and effect of temperature

Brazilian Journal of Medical and Biological Research

R. Gebhardt²  W. Doster²  U. Kulozik¹ 

[1] ,Technical University Munich Physics Department E13 Garching,Germany

关键词: Casein micelles;    High-pressure light scattering;    Pressure dissociation;   

DOI  :  10.1590/S0100-879X2005000800008

来源: SciELO

【 摘 要 】

Pressure-induced dissociation of a turbid solution of casein micelles was studied in situ in static and dynamic light scattering experiments. We show that at high pressure casein micelles decompose into small fragments comparable in size to casein monomers. At intermediate pressure we observe particles measuring 15 to 20 nm in diameter. The stability against pressure dissociation increased with temperature, suggesting enhanced hydrophobic contacts. The pressure transition curves are biphasic, compatible with a temperature (but not pressure)-dependent conformational equilibrium of two micelle species. Our thermodynamic model predicts an increase in structural entropy with temperature.

【 授权许可】

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