The yellow fever 17D vaccine virus: molecular basis of viral attenuation and its use as an expression vector

Brazilian Journal of Medical and Biological Research

R. Galler¹  M.s. Freire¹  A.v. Jabor¹  G.f. Mann¹ 

关键词: Flavivirus;    Yellow fever virus;    Attenuation;    Vaccine;    Gene expression;   

DOI  :  10.1590/S0100-879X1997000200002

来源: SciELO

【 摘 要 】

The yellow fever (YF) virus is the prototype flavivirus. The use of molecular techniques has unraveled the basic mechanisms of viral genome structure and expression. Recent trends in flavivirus research include the use of infectious clone technology with which it is possible to recover virus from cloned cDNA. Using this technique, mutations can be introduced at any point of the viral genome and their resulting effect on virus phenotype can be assessed. This approach has opened new possibilities to study several biological viral features with special emphasis on the issue of virulence/attenuation of the YF virus. The feasibility of using YF virus 17D vaccine strain, for which infectious cDNA is available, as a vector for the expression of heterologous antigens is reviewed

【 授权许可】

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