Biofilm formation by Rhodococcus equi and putative association with macrolide resistance

Pesquisa Veterinária Brasileira

Letícia T. Gressler¹  Agueda C. De Vargas¹  Mateus M. Da Costa¹  Fernando Jonas Sutili¹  Marcelo Schwab¹  Daniela Isabel B. Pereira¹  Luís Antonio Sangioni¹  Sônia De A. Botton¹ 

关键词: Antimicrobial resistance;    biofilm;    DAPI;    macrolides;    Rhodococcus equi;    Resistência antimicrobiana;    biofilme;    DAPI;    macrolídeos;    Rhodococcus equi;   

DOI  :  10.1590/S0100-736X2015001000003

来源: SciELO

【 摘 要 】

Abstract: Rhodococcus equi is a facultative intracellular pathogen, which cause severe pyogranulomatous pneumonia in foals and tuberculosis-like lesions in humans. Its ability to form biofilm was described in strains isolated from chronic diseases associated to treatment failures in humans. This study aimed to verify the biofilm formation by 113 R. equi isolated from equine samples (clinical and fecal) using two different methods (biofilm-culturing with and without additional glucose and epifluorescence microscopy). We also aimed to determine the efficacy of azithromycin, clarithromycin and erythromycin on R. equi in established biofilm. We found 80.5% (26/41) and 63% (58/72) biofilm-positive isolates, in fecal and clinical samples, respectively. The additional glucose increased the biofilm formation by R. equi fecal samples, but not by clinical samples. The antimicrobials tested herein were not able to eradicate R. equi in biofilm even at higher concentrations. This is the first study showing the biofilm formation by R. equi isolated from equine samples. Our findings indicate that R. equi biofilm-producers may be more resistant to the antimicrobials evaluated. Further studies are warranted to test this hypothesis.

【 授权许可】

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