Occurrence of enterotoxin-encoding genes in Staphylococcus aureus causing mastitis in lactating goats

Pesquisa Veterinária Brasileira

Daneelly H. Ferreira²  Maria Das Graças X. Carvalho²  Maria J. Nardelli²  Francisca G.c. Sousa¹  Celso J.b. Oliveira¹ 

[1] ,Universidade Federal de Campina Grande (UFCG) Centro de Saúde e Tecnologia Rural (CSTR) Inspeção e Tecnologia de Produtos de Origem AnimalPatos PB ,Brazil

关键词: Enterotoxin-encoding genes;    food poisoning;    goat milk;    Staphylococcus aureus;    Genes enterotoxigênicos;    intoxicação alimentar;    leite caprino;    Staphylococcus aureus;   

DOI  :  10.1590/S0100-736X2014000700004

来源: SciELO

【 摘 要 】

Staphylococcal enterotoxins are the leading cause of human food poisoning worldwide. Staphylococcus spp. are the main mastitis-causing agents in goats and frequently found in high counts in goat milk. This study aimed to investigate the occurrence of enterotoxin-encoding genes in Staphylococcus aureus associated with mastitis in lactating goats in Paraiba State, Brazil. Milk samples (n=2024) were collected from 393 farms. Staphylococcus aureus was isolated in 55 milk samples. Classical (sea, seb, sec, sed, see) and novel (seg, seh, sei) enterotoxin-encoding genes were investigated by means of polymerase chain reaction (PCR). From thirty-six tested isolates, enterotoxin-encoding genes were detected in 7 (19.5%) S. aureus. The gene encoding enterotoxin C (seC) was identified in six isolates, while seiwas observed in only one isolate. The genes sea, seb, sed, see, seg and seh were not observed amongst the S. aureus investigated in this study. In summary, S. aureus causing mastitis in goats can harbor enterotoxin-encoding genes and seC was the most frequent gene observed amongst the investigated isolates. This finding is important for surveillance purposes, since enterotoxin C should be investigated in human staphylococcal food poisoning outbreaks caused by consumption of goat milk and dairy products.

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