Expression of P-glycoprotein, multidrug resistance-associated protein, glutathione-S-transferase pi and p53 in canine transmissible venereal tumor

Pesquisa Veterinária Brasileira

Daniel G. Gerardi²  Mirela Tinucci-costa¹  Ana Carolina T. Silveira¹  Juliana V. Moro¹ 

[1] ,Universidade Federal do Rio Grande do Sul Faculdade de Veterinária Departamento de Medicina AnimalPorto Alegre RS ,Brazil

关键词: Transmissible venereal tumor;    P-glycoprotein;    multidrug resistance;    immunohistochemistry;    neoplasm;    dogs;    Tumor venéreo transmissível;    Glicoproteína-P;    Resistência à múltiplas drogas;    imuno-histoquímica;    neoplasias;    caninos;   

DOI  :  10.1590/S0100-736X2014000100012

来源: SciELO

【 摘 要 】

The overexpression of proteins P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP1), mutant p53, and the enzyme glutathione-S-transferase (GSTpi) are related to resistance to chemotherapy in neoplasms. This study evaluated the expression of these markers by immunohistochemistry in two groups of canine TVT, without history of prior chemotherapy (TVT1, n=9) and in TVTs presented unsatisfactory clinical response to vincristine sulfate (TVT2, n=5). The percentage of specimens positively stained for P-gp, MRP1, GSTpi and p53 were, respectively 88.8%, 0%, 44.5% and 22.2% in TVT1 and 80%, 0%, 80% and 0% in TVT2. In TVT1, one specimen presented positive expression for three markers and four specimens for two markers. In TVT2, three specimens expressed P-gp and GSTpi. In conclusion, the canine TVTs studied expressed the four markers evaluated, but just P-gp and GSTpi were significantly expressed, mainly at cytoplasm and cytoplasm and nuclei, respectively, either before chemotherapy as after vincristine sulfate exposure. Future studies are needed to demonstrate the function of these two markers in conferring multidrug resistance (MDR) or predict the response to chemotherapy in canine TVT.

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