Development and application of polymerase chain reaction test for detection of Conidiobolus lamprauges

Pesquisa Veterinária Brasileira

Marcelo M. Silveira²  Daphine A.j. Paula²  Maria C. Silva²  Leticia C. Pitchenin²  Raquel A.s. Cruz¹  Edson M. Colodel¹  Valéria Dutra²  Luciano Nakazato² 

[1] ,Universidade Federal de Mato Grosso Laboratório de Microbiologia Veterinária e Biologia Molecular Veterinária Cuiabá MT ,Brazil

关键词: Conidiobolus lamprauges;    diagnostic;    PCR;    sheep;    zygomycetes;    Conidiobolus lamprauges;    diagnóstico;    PCR;    ovinos;    zigomicetos;   

DOI  :  10.1590/S0100-736X2013001200009

来源: SciELO

【 摘 要 】

Conidiobolomycosis is a granulomatous disease caused by the fungus Conidiobolus spp. in humans and animals. Traditional technique for diagnosis of the disease is isolation of the agent associated with the presence of typical clinical signs and pathological conditions. The aim of this study was to describe the development of a specific polymerase chain reaction (PCR) test for Conidiobolus lamprauges to detect the fungus in clinical samples. Samples from suspected animals were collected and submitted to isolation, histopathological analysis and amplification by PCR. DNA from tissues was subjected to PCR with fungi universal primers 18S rDNA gene, and specific primers were designed based on the same gene in C. lamprauges that generated products of about 540 bp and 222 bp respectively. The culture was positive in 26.6% of clinical samples. The PCR technique for C. lamprauges showed amplification of DNA from fresh tissues (80%) and paraffin sections (44.4%). In conclusion, the PCR technique described here demonstrated a high sensitivity and specificity for detection of fungal DNA in tissue samples, providing a tool for the rapid diagnosis of C. lamprauges.

【 授权许可】

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