Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicles cultured in vitro

Pesquisa Veterinária Brasileira

Valdevane Rocha Araújo²  Isabel Bezerra Lima-verde²  Khessler Patrícia Olazia Name¹  Sônia Nair Báo¹  Cláudio Cabral Campello²  José Roberto Viana Silva¹  Ana Paula Ribeiro Rodrigues²  José Ricardo De Figueiredo² 

[1] ,Universidade Estadual do Ceará Campus do Itaperi Laboratório de Manipulação de Oócitos e Folículos Pré-AntraisFortaleza CE ,Brazil

关键词: TGF-β superfamily;    folliculogenesis;    germ cell;    growth factors;    goats;    Superfamília TGF-β;    foliculogênese;    célula germinativa;    fatores de crescimento;    caprinos;   

DOI  :  10.1590/S0100-736X2010000900010

来源: SciELO

【 摘 要 】

This study investigated the effects of bone morphogenetic protein 6 (BMP-6) on in vitro primordial follicle development in goats. Samples of goat ovarian cortex were cultured in vitro for 1 or 7 days in Minimum Essential Medium (control medium) supplemented with different concentrations of BMP-6. Follicle survival, activation and growth were evaluated through histology and transmission electron microscopy (TEM). After 7 days of culture, histological analysis demonstrated that BMP-6 enhanced the percentages of atretic primordial follicles when compared to fresh control (day 0). Nevertheless, BMP-6 increased follicular and oocyte diameter during both culture periods. As the culture period progressed from day 1 to day 7, a significant increase in follicle diameter was observed with 1 or 50ng/ml BMP-6. However, on the contrary to that observed with the control medium TEM revealed that follicles cultured for up to 7 days with 1 or 50ng/ml BMP-6 had evident signs of atresia. In conclusion, this study demonstrated that BMP-6 negatively affects the survival and ultrastructure of goat primordial follicles.

【 授权许可】

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