Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media

Pesquisa Veterinária Brasileira

Paula Ristow²  Marlei Gomes Silva²  Leila De Souza Fonseca²  Walter Lilenbaum¹ 

[1] ,Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Rio de Janeiro RJ ,Brazil

关键词: Paratuberculosis;    bovine;    diagnosis;    mycobacterium;    Paratuberculose;    bovinos;    diagnóstico;    mycobacterium;   

DOI  :  10.1590/S0100-736X2006000100001

来源: SciELO

【 摘 要 】

Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map.

【 授权许可】

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