Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media

Pesquisa Veterinária Brasileira

Fonseca, Leila de Souza¹  Lilenbaum, Walter²  Ristow, Paula¹  Silva, Marlei Gomes¹ 

[1] Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Universidade Federal Fluminense, Niterói, Brazil

关键词: Paratuberculosis;    bovine;    diagnosis;    mycobacterium.;   

DOI  :  10.1590/S0100-736X2006000100001

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map. Index

【 授权许可】

CC BY-NC