Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate

Pesquisa Veterinária Brasileira

Patricia Elena Fernández¹  Héctor Ramón Sanguinetti¹  Enrique Leo Portiansky¹  Claudio Gustavo Barbeito¹  Eduardo Juan Gimeno¹ 

[1] ,National University of La PlataLa Plata

关键词: Bovine prostate;    cell markers;    immunohistochemistry;    oestrogen;    Próstata bovina;    marcadores de celulares;    imunohistoquímica;    estrógenos;   

DOI  :  10.1590/S0100-736X1999000300008

来源: SciELO

【 摘 要 】

The immunodetection of diverse cell markers was evaluated in prostatic samples from bullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes.

【 授权许可】

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