Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

Química Nova

Xikai Shu²  Mei Wang²  Daicheng Liu²  Daijie Wang¹  Xiaojing Lin¹  Jianhua Liu¹  Xiao Wang¹  Luqi Huang¹ 

[1] ,Shandong Normal University College of Life Science ,China

关键词: artichoke;    HSCCC;    polyphenols;   

DOI  :  10.1590/S0100-40422013000600017

来源: SciELO

【 摘 要 】

An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR).

【 授权许可】

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