Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

Química Nova

Huang, Luqi¹  Lin, Xiaojing¹  Shu, Xikai¹  Shandong Normal University, China¹  Liu, Jianhua¹  Shandong Academy of Sciences, Shandong, China¹  Wang, Daijie¹  Wang, Mei¹  Liu, Daicheng¹  Wang, Xiao¹ 

关键词: artichoke;    HSCCC;    polyphenols.;   

DOI  :  10.1590/S0100-40422013000600017

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR).

【 授权许可】

Unknown